鼻咽癌中抗凋亡基因bcl-2的表达及其与EB病毒的关系

EXPRESSION OF ANTI APOPTOSIS bcl 2 GENE AND RELATIONSHIP BETWEEN bcl 2 AND EB VIRUS IN NASOPHARYNGEAL CARCINOMA

为了探讨鼻咽癌（ＮＰＣ）中抗凋亡基因ｂｃｌ－２的表达及其与ＥＢ病毒的关系。用免疫组化方法对曾用ＥＢ病毒探针进行原位杂交的ＮＰＣ标本再进行ｂｃｌ－２检测。结果：非角化型鳞状细胞癌５４例，阳性５１例，阴性３例；角化型鳞状细胞癌１２例，阳性３例，阴性９例；腺癌３例，阳性１例，阴性２例；ＮＰＣ淋巴结转移性鳞状细胞癌１１例，阳性９例，阴性２例；非ＮＰＣ淋巴结转移性鳞状细胞癌１０例，阳性２例，阴性８例。ＮＰＣ各组织学类型中ｂｃｌ－２的表达与ＥＢ病毒原位杂交表达的结果相似。提示ｂｃｌ－２基因表达与ＮＰＣ组织学类型关系密切，非角化型大部分为阳性表达，角化型大部分为阴性表达，并提示ＥＢ病毒在ＮＰＣ的发生机制上可能与抑制细胞凋亡的基因有协同作用。

In order to investigte the relationship between bcl 2 and EB virus,69 cases of nasopharyngeal carcinoma(NPC).He expression of anti apoptosis bel 2 gene and who had been examined for EB virus by in situ hybridization(ISH),were studied for the expression of bcl 2 by SP methods.The results showed that 51 of 54 cases of non keratinizing squamous cell carcinoma were postive;3 of 12 of squamous cell carcinoma were postive;1 of 3 cases of adenocarcinoma was positive;9 of 11 cases of NPC with.LN metastasis were positive.2 of 10 cases of non NPC with LN metastasis were positive.The expressions bcl 2 in various histological types of NPC were similar to those of EB virus by ISH.The results suggest that there is a close relationship between the expression of bcl 2 and the histological types of NPC;most of the non keratinizing squamous cell carcinoma are positive for bcl 2,while most of the squamous cell carcinoma negative.The role of EB virus in the pathogenesis of NPC may be related to the inhibition of cellular apoptosis.