美洲商陆组培快速繁殖

Tissue Culture and Rapid Propagation of Phytolacca americana

目的:探索美洲商陆组培快速繁殖技术。方法:以无菌苗作外植体。结果:初代培养的较粗壮茎段为快速繁殖的最佳外植体,初代培养基为MS+NAA0.2 mg/L+6-BA1.0 mg/L;丛生芽最佳增殖培养基为MS+NAA0.2mg/L+6-BA2.0 mg/L,最适生根培养基为1/2MS+NAA0.4 mg/L。结论:采用美洲商陆无菌苗茎段诱导丛生芽可提高繁殖系数,为大量种植提供种苗。

Objective： To explore the technique of rapid propagation for Phytolacca americana. Methods： Aseptic seedling were used as explants. Results：The best explants were the stems from strong aseptic seedling. The optimal culture media were MS ＋ NAA （0. 2 mg/L） ＋6-BA（ 1. 0 mg/L） for primarily culture, MS ＋ NAA（0. 2 mg/L） ＋6-BA（2. 0 mg/L） for the induction of clustered shoots 1/2MS with NAA 0. 4 mg/L for rooting. Conclusion ：The propagating coefficient of Phytolacca americana can be improved by inducing the clustered shoots from aseptic seedling.