巴戟天抗抑郁提取物含量测定及微量元素分析

Determination Content of the Antidepressant Extraction and Analysis the Trace Elements from Morinda officinalis

目的:研究药材巴戟天提取物(寡糖Ⅰ)的糖、蛋白质含量以及微量元素的分析。方法:采用苯酚-硫酸法测定总寡糖的含量,考马斯亮蓝染色法测定蛋白质含量,ICP-AES法分析其中的微量元素。结果:该提取物含总寡糖量为89.4%,总提取物中蛋白质含量为0.19%,经过D-941阴离子树脂柱层析富集脱蛋白后未测出含有蛋白质,微量元素测定其含有Zn、Fe、Ca等十二种微量元素,未检测出As、Cd、Pb等有害元素。结论:该提取物中含具有抗抑郁作用的高纯度植物寡糖。

Objective ： To study on the antidepressive extraction and determinated the content of the oligosaccharides Ⅰ , the proteins and the trace elements from Morinda officinalis. Methods ： We analyzed the content of the total oligosaccharides, the proteins and the trace elements by Phenol-Sulferic acid, Coomassie brilliant blue and inductive coupling plasm atomic emission spectrometer（ ICPAES） methods, respectively. Results： The percent of the oligosaccharides and the proteins were 89.4% and 0. 19% in the extraction from M. officinalis, respectively. The total actively oligosaecharides Ⅰ were subjected to the D-941 ion column chromatography to give the extraction in which hardly and protein were determined. 12 kinds of trace element including Zn, Fe, Ca ect. in the extraction （the oligosaccharides Ⅰ ） were determined by ICP-AES without the noxious trace elements including As, Cd, Pb ect. Conclusion： The antidepressive activity extraction（ the oligosaccharides Ⅰ ） was a highly pure and vegetal oligosaccharides.