白藜芦醇对多种呼吸道病毒作用体外实验研究

In vitro Experimental Study on the Effect of Resveratrol against several Kinds of Respiroviruses

目的:观察白藜芦醇对多种呼吸道病毒导致细胞病变的抑制作用,为进一步探讨白藜芦醇的抗病毒机理积累基础。方法:分别接种甲1型流感病毒FM1株、呼吸道合胞病毒long株、腺病毒7型、鼻病毒R14型于MD-CK、A549、HEp-2、MRC-5细胞,采用细胞病变抑制法(CPE),观察白藜芦醇体外对多种呼吸道病毒作用。结果:白藜芦醇在无毒浓度120μg/m l下,对腺病毒7型所致的细胞病变有抑制作用,而对甲1型流感病毒FM1株、呼吸道合胞病毒long株、鼻病毒R14型均未见抑制作用。结论:白藜芦醇具有体外抑制腺病毒7型作用,其对DNA病毒作用机理值得进一步探讨。

Objective：To observe the cytopathogenic inhibitory effect of resveratrol on vary respiroviruses and explore the mechanism of resveratrol against viruses. Methods ： MDCK, A549, HEp-2 cell and MRC-5 were infected with Influenza virus type A FM1 strain, rhinovirus type R14, RS virus, AD virus type 7 separately, and the antiviral activity of resveratrol were observed. Results：Resveratrol significantly inhibited cytopathogenie effect of AD virus type 7 at the concentration 120μg/ml. No significant cytopathogenic effect of Resveratrol inhibiting Influenza virus type A FM1 strain, Rhinovirus type R14, RS virus on separate cells was observed. Conclusion：It is concluded that resveratrol is effective on inhibiting AD virus type 7 in vitro, however, its mechanism is needed for further study.