甲状腺片诱导的甲亢阴虚证大鼠模型的制备

Thyroid-induced Hyperthyroidism Yin-deficiency Model of Rats

目的:建立甲亢阴虚证大鼠模型。方法:取SD大鼠雌雄,分别随机分为4组,共8组,每组6只,分别为空白对照组、甲状腺片生理盐水混悬液低、中、高剂量组。空白对照组灌胃给予等体积的生理盐水,各给药组组分别给予浓度为2.5、5、10 mg/kg的甲状腺片生理盐水混悬液,1次/d,连续给药30 d。结果:给药组与空白对照组比较,除高剂量组雌性大鼠血清T3、T4、FT3、FT4值显著升高(P<0.01),血清TSH值显著降低(P<0.05)外,其余组均无显著性差异(P(0.05)。说明高剂量组雌性大鼠造模成功。结论:灌服甲状腺片混悬液10 mg/kg,连续给药30 d的雌性大鼠甲亢阴虚证大鼠模型制备成功。

Objective： To establish a rat model of hyperthyroidism Yin-deficiency. Methods： SD rats separate male and female were randomly divided into four groups, a total of eight groups, each with six. The first group for the control group, 2 to 4 for the experimental drug-treated group, including thyroid-low, medium and high-dose group. In addition to the first group lavage volume of saline, the other groups were given the concentration of 2.5,5, 10mg/kg-saline suspension of the thyroid. Once a day, 30 days for delivery. Results ： Compared with the control group, with the exception of Group 4 female rats serum T3 , T4 , FT3 , FT4 values were significantly higher （ P 〈0.01 ）, serum TSH was significantly lower （ P 〈0. 05 ）, the other treatment groups had different values no significant difference （ P 〉 0. 05 ）. Group 4 that female rats modeling was success. Conclusion ： Administration of thyroid-Suspension 10 mg/ kg for 30 d, female rats hyperthyroidism Yin-deficiency rat model is success.