浙江省柑橘绿霉病菌对嘧菌酯的敏感性研究

Sensitivisty of Penicillium digitatum in Zhejiang to the Strobilurin Fungicide Azoxystrobin

采用孢子萌发法和生长速率法测定了2000～2006年间采自浙江衢州、杭州、金华、丽水的65个柑橘绿霉病病菌Penicillium digitatum菌株对嘧菌酯的敏感性。结果表明：嘧菌酯对供试菌株孢子萌发和菌丝生长的EC50值均呈单峰分布，分别介于0．0201～0．2600μg/mL和0．0053—0．0794μg／mL之间，平均值分别为0．0426μg／mL和0．0250μg／mL。敏感性频次分析结果表明，该65个菌株孢子萌发和菌丝生长对嘧菌酯的敏感性频率分布均符合正态分布，其EC50平均值0．0426±0．0304μg／mL和0．0250±0．0129μg/mL可分别作为柑橘绿霉病菌孢子萌发和菌丝生长对嘧菌酯的敏感基线。

Sixty-five isolates of Penicillium digitatum were collected from Quzhou, Hangzhou, Jinhua and Lishui in Zhejiang Province during 2000 to 2006, where strobilurin fungicide azoxystrobin had never been used to control green mold of postharvest citrus. The sensitivities of the isolates to azoxystrobin were assayed by the methods of conidial germination and mycelial linear growth. The results demonstrated that the EC50 values for conidial germination and mycelial growth ranged from 0.020 1 to 0.2600 μg/mL and 0.0053 to 0.0794 μg/mL, and averaged 0.0426 and 0.0250μg/mL, respectively. The frequency distributions of EC50 values of the 65 isolates were normally distributed for both conidial germination and mycelial growth. Thus, the mean EC50 values 0.042 6±0.030 4 and 0. 025 0 ±0. 012 9 μg/mL of the 65 isolates could be used as the sensitivity baselines for conidial germination and mycelial growth of P. digitatum to azoxystrobin, respectively.