子宫肌瘤患者血清雌孕激素的测定及雌激素受体和孕激素受体在子宫肌瘤的表达及意义

Expression and significance of ER and PR in uterine leiomyomas

目的探讨子宫肌瘤患者血清雌激素(E)、孕酮(P)水平以及子宫肌瘤组织和邻近正常肌组织中雌激素受体(ER)、孕激素受体(PR)的表达及意义。方法采用放射免疫法测定子宫肌瘤患者血清E、P水平。并采用免疫组织化学法测定子宫肌瘤组织和邻近正常肌组织中ER、PR的表达情况。结果在增生期和分泌期,子宫肌瘤患者血清E、P水平较正常育龄期妇女均略有上升,但差异无显著性(P>0.05)。在子宫肌瘤和正常平滑肌组织中,ER阳性细胞数在增生期均多于分泌期(P<0.01),PR阳性细胞数在分泌期均多于增生期(P<0.01)。结论E、P大量结合于子宫肌瘤局部的ER、PR,使肌瘤局部呈现高激素状态,这可能是子宫肌瘤细胞不断增生的重要原因。

Objective To explore the etiology of uterine leiomyomas by detecting the expression of estrogen receptor （ ER）, progesterone receptor （PR） and the level of serum estrogen and progesterone. Methods The levels of serum estrogen and progesterone were detected in all cases. The expression of ER and PR in leiomyoma and normal matched myometrium was detected by staining. Results In proliferative and secretory phases, the levels of serum estrogen and progesterone were higher in patients with uterine leiomyornas than those in normal woman. However, there were no significant differences （ P 〉 0.05 ）. The number of ER positive cells was significantly higher in proliferative phase than that in secretory phase （ P 〈0.01 ） ; the number of PR positive cells was significantly higher in secretory phase than that in proliferative phase （ P 〈 0.01 ）. Conclusion Localized higher levels of estrogen and progesterone resulted from the conjugation of estrogen and progesterone with ER and PR in uterine leiomyoma may be the important etiology of uterine leiomyoma.