测定K562细胞培养液中苦参碱浓度变化的反相离子对HPLC研究

Research for determining the concentration of matrine in K562 culture-medium by reversed phase ion-pair HPLC

本文建立了一种用于测定苦参碱含量的反相离子对高效液相色谱法（ｉｏｎ－ｐａｉｒＨＰＬＣ）。该法比较准确、可靠，分离效果好，灵敏度高。可用于细胞培养液中苦参碱的分离测定及鉴定。结合样品前处理，作者运用此法对苦参碱在Ｋ５６２细胞培养液中的浓度变化情况进行了初步分析。发现在我们研究的实验条件下，Ｋ５６２细胞培养液中苦参碱的浓度先呈线性下降趋势，以后１－３天则浓度维持不变。

A reversed phase ion - pair high performance liquid chromatographic method was established for determining the concentration of matrine. The method was accurate and reliable with fine resolution and high sensitivity Matrine in medium can be seperated, determined and identified by this method. After extracted form the medium,the matrine was analysed preliminarily.We found that the concentration of matrine had a linear decline in the first day and was invariable during two days after that.