透明质酸对伤口愈合调控作用的实验研究

Modulating effects of exogenous hyaluronic acid on wound healing:an experimental study

为观测透明质酸（ＨＡ）对成年动物伤口愈合调控作用和动态变化，探索其作用机理，本研究采用大鼠开放性创伤模型，局部应用ＨＡ后于伤后３．７．１４天取肉芽组织，标本经光镜、电镜检查和羟脯氨酸测定，结果显示：局部应用ＨＡ后，伤后３天肉芽组织中新生毛细血管较少，伤后７天成纤维细胞出现严重褪变，而其羟脯氨酸含量也显著低于对照组，伤后１４天形成的胶原纤维排列较整齐。提示ＨＡ对成年动物伤口愈合具有调控作用并对于减少或控制疤痕形成具有潜在意义。

To investigate the modulating effects of exogenous hyaluronic acid(HA) on adult wound healing and its dyanamic process, the excisional cutaneouos wounds on dorsum in 62 rats were made and exogenoua HA was applied topically. Granulating tissue specimens were harvested at 3, 7 and 14 days after wounding for light microscopic, transsmission electron microscopic examination and hydroxyproline(HYP) determination. lt was demonstrated that after topical application of cxogenous HA:at 3rd day postwounding angiogenesis was remarkabaly less than that irt control group, but fibrubasts degenerated aeriously and the contents of HYP was significantly lower than th at in control at 7th day; 14 days after wounding, the depositcd collagen in HA group was more well-organized than that in control group.it is concluded that the modulating effects was more effective in early. period and less in later stage.It is sugggested that exogenous HA might have potential value to lesen or. control scar formation.