血管活性肠肽受体在人结肠癌细胞膜上的表达

Expression of Vasoactive Intestinal Peptide Receptor in Colonic Carcinoma Cell Membranes in Men

为评价血管活性肠肽受体在人结肠癌细胞膜膜片上表达的意义，并探讨该受体特性与肿瘤组织学特征间的关系，作者用碘标记血管活性肠肽，经放射配体结合分析法测定１２例结肠癌标本细胞膜片上血管活性肠肽的受体位点数。结果显示：结肠癌细胞膜片上的血管活性肠肽受体位点数为２．６６±３．８４ｐｍｏｌ／ｍｇ膜蛋白，明显低于其毗邻的正常细胞膜膜片上该受体的位点数１０．５４±１７．９９ｐｍｏｌ／ｍｇ膜蛋白。癌细胞受体密度与细胞分化程度关系不明显。由此提示调控结肠癌血管活性肠肽受体表达。

To evaluate the expression of vasoactive intestinal peptide receptor in colonic carcinoma cell membranes in men and to assess the relationship between the receptor characteristics and the histopathologic features,the authors labelled vasoactive intestinal peptide and measured vasoactive intestinal peptide receptor sites with radioligand bind assay on 12 specimens from colon cancer and its adjacent normal tissues.The number of the vasoactive intestinal peptide receptor sites of colon cancer was significantly smaller than that of the adjacent normal tissues(2.66±3.84 pmol/mg versus 10.54±17.99pmol/mg,and the number of the receptor sites of colon cancer was not well related to the degrees of cancer differentiation.This study has laid the basis for the regulation of the receptorligand binding to inhibit the growth of colon cancer.