全血无酚法制备染色体DNA

Preparation of Chromosomal DNA from Whole Blood Without Use of Phenol

报道一种全血无酚法制备染色体ＤＮＡ技术。其特点是：不预先分离白细胞或细胞核，用全血直接提取ＤＮＡ；用饱和ＮａＣｌ代替经典法中的酚／氯仿有机溶剂。所制备的染色体ＤＮＡ２６０／Ａ２８０比值为１．８０～１．８５。每毫升外周血可获得２０μｇ～２８μｇＤＮＡ。该法是一种简便、安全。

We describe in this paper a simple and efficient procedure for preparing peripheral blood DNA without preisolation of white blood cells.The method also avoids the hazard of organic solvent extraction.Routinely it yields 20 to 28μg of chromosomal DNA from per ml whole blood.The A260/A280 ratio for DNA sample is 1.80 to 1.85.The undigested DNAs migrate above the 21kb pair,while the endonucleasedigested samples appear characteristically in the smear form.These data illustrate the consistency and quality of the chromosomal DNA isolated using this procedure.The DNA prepared by this method was used for analysis of βthalassemia mutation and prenatal diagnosis in our laboratory.