黄芪三七有效成分配伍抗动脉粥样硬化作用的实验研究

Empirical Study of Effective Elements Compatibility of Astragalus and Panax Notoginseng on Anti-atherosclerosis

目的:探讨黄芪、三七有效成分槲皮素(Que)和三七总皂甙(tPNS)对动脉粥样硬化(AS)模型大鼠血清白细胞分化抗原40的配体(CD40L)表达的影响。方法:高脂饮食建立AS大鼠模型。造模成功后药物干预4周,用酶联免疫吸附试验(ELISA)测各组血清CD40L表达。结果:高脂饮食能诱发大鼠形成AS模型。以槲皮素三七总皂甙3:1组治疗效果最佳,与各组血清CD40L的表达量相比,有统计学意义(P(0.05)。结论:两者不同比例配伍组方可以下调血清CD40L的表达。檞皮素三七总皂甙3:1组方疗效最佳。

Objective： To explore the effect of quercetin and total panax notoginseng saponins on CD40L expression in atherosclerotic model rat. Method： Healthy male Wistar rats were fed up with high fat diet for 12weeks to make atherosclerosis model. After 3 months, different medicines were given to the rats in different groups respectively, and ligustrazine was given to the rats in control group by gastrogavage for 4 weeks. Finally, CD40L expression in serum was determined by ELISA method. Result： High fat diet could induce rat to come into being athero- sclerosis model. CD40L expression in 3：1 treatment group showed statistical significance comparing with other groups（P 〈0.05）. And Quercetin and total Panax Notoginseng Saponins in 3：1 treatment group showed better effect. Conclusion ：Different proportional compatibility combinations can down-regulate CD40L expression. And 3：1 treatment group shows the best effect on decreasing CD40L expression.