培养基组分对沙打旺(Astragalus adsurgens Pall)组培根增殖的影响及其培养滤液提取物的化感活性(英文)

Effect of growth medium composition on the propagation of cultured milk vetch(Astragalus adsurgens Pall) roots and the allelopathic activity of extracts from the culture filtrate

采用L9(315)正交设计,研究了B5培养基营养组分对沙打旺组培根增殖的影响;并采用玻璃皿滤纸培养法,对其培养滤液提取物进行生物测定以验证沙打旺组培根的化感活性。结果显示:培养基的所有营养组分中,Fe2+对沙打旺组培根增殖的影响最大,蔗糖、H2PO4+、Mg2+、Mn2+、Cu2+、Zn2+、BO33-、Co2+、I-、C8H12ClNO3+C12H18Cl2N4OS+C6H5O2N+C6H12O6的影响次之,氮、Ca2+、MoO42-和NAA的影响最小。根据不同养分条件下沙打旺组培根干重的极差分析,筛选出适宜沙打旺组培根快速增殖的优化培养基。培养滤液提取物的生物测定结果表明沙打旺组培根培养过程中可能产生化感物质;化感作用强度的差异预示营养胁迫可能影响其化感物质的产生。研究为沙打旺组培根再生与繁殖提供一定依据,并揭示养分条件可能是该植物表达化感作用的影响因素。

The objective of this research was to determine effects of growth medium composition on the propagation of cultured milk vetch （Astragalus adsurgens Pall） roots. An orthogonal design was used to test multiple components in the growth medium. A second objective was to measure allelopathic activities of cultured milk vetch roots by conducting a bioassay using filtered extracts from the growth medium after culturing the roots. The results indicated that among all the nutritional components, Fe2＋ had the greatest effect on the propagation of cultured milk vetch roots; the effects of sucrose, H2PO^4＋、Mg^2＋、Mn^2＋、Cu^2＋、Zn^2＋、BO3^3-、Co^2＋、I^-、C8H12ClNO3＋C12H18Cl2N4OS＋C6H5O2N＋C6H12O6 were intermediate; and the effects of nitrogen, Ca^2＋ , MoO4^2- and NAA were lowest. A recommendation was made regarding the optimum nutrient content of growth medium for the propagation of cultured milk vetch roots. A bioassay using filtered extracts from the growth medium indicated that milk vetch roots might have produced allelopathic chemicals during their culture. Differences in the degree of allelopathic effects suggest that nutrient stress may influence the production of allelopathic chemicals. This study provides a basis for the improvement of milk root regeneration and propagation and suggests that nutrition may influence the production of allelopathic chemicals by this plant.