人髓样细胞触发性受体-1在急性胆管炎的表达及意义

Expression and significance of human triggering receptor on myeloid cells-1 in acute cholangitis

目的观察人髓样细胞触发性受体（TREM）-1在急性胆管炎患者中表达的规律及其临床意义，探讨TREM-1在急性胆管炎中与肿瘤坏死因子（TNF）-α，CRP的相关性。方法分别收集34例急性胆管炎患者（AC），24例急性重症胆管炎（ACST）患者和60例正常人外周血白细胞，以半定量逆转录一聚合酶链反应（RT-PCR）检测中性粒细胞中TREM-1的mRNA表达；免疫细胞化学检测TREM-1的蛋白表达。结果对照组TREM-1的mRNA半定量比值为0．458±0．043。AC和ACST组治疗前分别为0．809±0．044、0．981±0．089，术后24h为0．785±0．077、0．954±0．072，48h为0．565±0．009、0．719±0．047，ACST的TREM-1表达水平明显高于AC（P〈0．05）。免疫细胞化学镜下观察结果与RT-PCR结果一致。TREM-1与TNF-α，CRP表达水平高度正相关（r1=0．719，r2=0．701，P〈0．01）。结论人TREM-1在急性胆管炎发病初表达增高，与TNF-α，CRP表达高度正相关。提示TREM-1在急性胆管炎的发生发展中起重要作用。

Objective To investigate the expression of human triggering receptor on myeloid cells-1 （TREM-1） and its regularity or clinical significance in patients with acute cholangitis,and discuss the relationship of TREM-1 ,TNF-α and CRP in acute cholangitis. Methods Peripheral blood of 24 patients with ACST,34 patients with AC and 60 health adults were collected respectively. TREM-I mRNA and protein was delected by semi-quantitative RT-PCR and immunocytochemistry respectively. Results The values of TREM-1/β-actin in control group was 0. ,158 ± 0. 043, while those in AC and ACST group were 0. 809 ± 0.044 and 0.981±0.089 before therapy,and 0. 785 ± 0. 077,0.954± 0.072 at 24 h,0. 565 ± 0. 009,0. 719 ±0.047 at 48 h, respectively. The level of TREM-1 express in ACST was higher than in AC （ P 〈 0.05 ）. Immunocytochemistry revealed the results similar to those of RT-PCR. TREM-1 expression was closely related with TNF-α,CRP expression （r1 = 0. 719 ,r2 = 0. 701 ,P 〈 0.01 ）. Conclusion Expression of human TREM-1 was up-regulated obviously in patients with AC and ACST in the early stage,indicating that TREM-1 might play an important role in the development of acute cholangitis.