组织蛋白酶B和趋化因子受体4在乳头状甲状腺癌的表达及在侵袭转移中的作用

The expression of cathepsin B and CXC chemokine receptor 4 in papillary thyroid carcinoma and their contribution to invasion and metastasis of papillary thyroid carcinoma

目的观察组织蛋白酶B（CB）、趋化因子受体4（CXCR4）mRNA和蛋白在乳头状甲状腺癌（PTC）中的表达，探讨其在乳头状甲状腺癌侵袭和转移中的作用。方法采用逆转录一聚合酶链反应（RT-PCR）及免疫组织化学SP法检40例PTC，10例正常甲状腺中CB、CXCR4mRNA和蛋白的表达。结果CB、CXCR4mRNA和蛋白在正常甲状腺中均为阴性表达；CB、CXCR4 mRNA在PTC中阳性表达率82．5％（33／40）、80％（32／40）；CB、CXCR4蛋白在PTC中阳性表达率65％（26／40）、60％（24／40）；CB，CXCR4mRNA表达与蛋白表达之间呈显著正相关（r=0．352，P〈0．05；r=0．357，P〈0．05），且均与侵润深度，淋巴结转移密切相关（P〈0．05）；CB，CXCR4蛋白表达呈正相关（r=0．471，P〈0．05）。结论CB、CXCR4在乳头状甲状腺癌的侵袭和转移中发挥促进作用。

Objective To observe the expression of cathepsin B （ CB ） and CXC chemokine receptor 4 （CXCR4） mRNA and protein in papillary thyroid carcinoma （PTC） and explore their contribution to invasion and metastasis of papillary thyroid carcinoma. Methods The expression of CB and CX- CR4 mRNA and protein in 40 cases of PTC and 10 cases of normal thyroid was detected by reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemical SP method. Results The expression of CB and CXCR4 mRNA and protein was negative in normal thyroid ; The positive expression rate of CB and CXCR4 mRNA in PTC was 82.5% （33/40） and 80% （32/40） respectively;The positive ex- pression rate of CB and CXCR4 protein in PTC was 65% （26/40） and 60% （24/40） respectively. There was a positive correlation between CB and CXCR4 mRNA and protein （ r = O. 352, P 〈 0.05 ; r = 0. 357, P 〈 0.05 ）. The expression of CB and CXCR4 mRNA and protein was significantly related to depth of invasion and lymph node metastasis （ P 〈 0.05 ）. There was a positive correlation between the expression of CB and CXCR4 protein （r = 0.471 ,P 〈 0.05 ）. Conclusion CB and CXCR4 play a positive role in invasion and metastasis of papillary thyroid carcinoma.