mTOR基因在人肝癌组织中的表达

Expression and significance of mTOR gene in human hepatocellular carcinoma

目的观察人肝癌组织哺乳类动物雷帕霉素靶蛋白（mTOR）基因的表达。方法应用半定量逆转录-聚合酶链反应（RT-PCR）方法检测10例人中晚期肝癌及其癌旁正常组织中mTORmRNA的相对表达量，并采用Westernblot检测上述组织中mTOR蛋白产物表达。结果90％（9／10）的肝癌组织中mTORmRNA和蛋白表达增强，而癌旁组织中80％（8／10）该基因表达缺失和蛋白表达减少，两者差异有统计学意义（P〈0．05）。结论中晚期肝癌中mTOR表达处于活化状态，可能在肝癌的发生、发展过程中发挥重要作用。

Objective To investigate the expression of mammalian target of rapamycin （mTOR） gene in human hepatoeellular carcinoma （hHCC）. Methods The samples were obtained from 10 cases of hHCC tissues. Semi-quantitative RT-PCR and Western blot were used to detect the InTOR mRNA and protein expression in 10 samples and adjacent noncanerous tissues. Results mTOR mRNA and protein expression was detected in 90 % of hHCC tissues and of 20% adjacent noncanerous tissues （ P 〈 0.05 ）. Conclusion mTOR activation might be in hHCC. mTOR gene may play some roles in the pathogenesis of HCC.