静水压对软骨细胞凋亡和增殖细胞核抗原表达的影响

Effects of static pressure on apoptosis of chondrocytes and the PCNA expression

目的探讨静水压对软骨细胞凋亡和增殖的影响及肿瘤坏死因子（TNF）-α和自细胞介素（IL）-1β与软骨细胞凋亡的关系。方法兔膝关节软骨细胞放置在压力装置中采用不同持续时间（0、4、16、24h）和不同大小静水压力刺激（0、20、40、70kp）后观察其凋亡及PCNA表达，测定相应细胞培养基中TNF-α和IL-1β的值。结果软骨细胞受到不同大小静压力后，作用早期细胞凋亡显著（P〈0．05），随时间延长凋亡值出现不同的变化。PCNA的表达在不同的压力下和对照组比较均有差异有统计学意义（P〈0．05）。TNF-α可重复双因素分析，时间和压力的交互作用（F=6．90，P〈0．01）。IL-1β可重复双因素分析，时间和压力的交互作用（F=5．80，P〈0．01）。结论软骨细胞在持续高压力应力下凋亡增加，增殖减少；在持续低压力时出现相反的结果。软骨细胞的凋亡和增殖和TNF-α和IL-1B变化有一定的相关性。

Objective To investigate the effects of static pressure on apoptosis and proliferation of condrocytes and the correlation between IL-1β and TNF-α and the apoptosis of chondrocytes. Methods The chondrocytes of rabbits were cultivated in different static pressure conditions （20,40,70 kp） for different durations （0,4,16,24 h） respectively,then the cells were collected. The apoptosis of chondrocytes was assayed by FCM. The expression of PCNA was detected. The IL-1β and TNF-α of culture medium were evaluated at same time. Results The apoptosis of ehondrocytes was increased after mechanical stimuli whatever its magnitude. With prolongation of time ,the apoptosis index had different changes （P 〈 0.05 ）. The proliferation were inhibited at first 4 hours （ P 〈 0.05 ） , and then increased rapidly after the following time. TNF-α was increased with the time lasting at 40 kp and 70 kp, and it correlated to ehondrocyte apoptosis. Tile IL-1β was decreased with the time lasting at 70 kp, and it negative correlated apoptosis of chondrocyte,we also found the IL-1β decreased when enhanced pressure on 24 h and it correlated the PCNA express at 24 h. Conclusion So we could conclued that the number of chondrocytes were decreased at high static Dressure and long effect time.