小檗碱与梓醇及其配伍对胰岛素抵抗3T3-L1脂肪细胞葡萄糖转运子4蛋白及C-Cb1相关蛋白表达的影响

Effect of catalpol,berberine,and their combination on expression of Glut4 protein and C-Cb1 associated protein in insulin resistant 3T3-L1 adipocytes

目的观察梓醇与小檗碱及其配伍对胰岛素抵抗3T3-L1脂肪细胞葡萄糖消耗及这一过程中葡萄糖转运子4(Glut4)和C-Cb1相关蛋白(CAP)表达的影响。方法采用高糖联合高胰岛素诱导3T3-L1脂肪细胞产生胰岛素抵抗(IR),分别给予小檗碱、梓醇、小檗碱+梓醇、盐酸罗格列酮进行干预,以葡萄糖氧化酶法检测培养液中葡萄糖消耗量,以Western Blotting法检测Glut4和CAP蛋白的表达。结果与模型组相比,小檗碱能增加培养液中葡萄糖的消耗,但对Glut4蛋白的表达无影响;梓醇、小檗碱+梓醇均能显著增加培养液中葡萄糖的消耗,并使细胞中Glut4蛋白的表达增强,且小檗碱+梓醇组的效应优于梓醇组及小檗碱组;与模型组相比,小檗碱与梓醇及其配伍对CAP的表达没有显著性影响。结论小檗碱、梓醇及其配伍能改善IR 3T3-L1脂肪细胞的胰岛素活性,其作用机制与罗格列酮不同。

Objective To observe the effect of catalpol, berberine, and protein and C-Cb1 associated protein （CAP） on 3T3-L1 adipocytes induced with insulin resistance （IR）. Methods IR Model of adipocyte was induced with the combination of high glucose and hyperinsulinism, cells were treated with rosiglitazone, catalpol, berberine, and combination of catapol and berberine. Glucose consumption of cells incubated with corresponding medicine was assayed with glucose oxidase method, Glut4 protein and CAP expression were detected with Western Blotting. Results Glucose consumption and Glut4 protein expression were significantly increased in catalpol group and combination of berberine and catalpol group compared to model group. At the same time, the capacity is more significant in the combination of berberine and catapol group; Berberine group could improve glucose consumption, but it had no effect on the expression of Glut4. compared with model group, these three groups had no significant effect on the expression of CAP. Conclusion Berberine, catapol, and combination of berberine and catalpol could improve the insulin sensity of 3T3-L1 adipocytes induced with IR, but the machanism might be different from that of rosiglitazone.