羟基积雪草苷对小鼠急性肝损伤的保护作用

Protection of madecassoside on mice with acute liver injury and its mechanism

目的研究羟基积雪草苷对小鼠急性肝损伤的保护作用及其初步机制。方法建立四氯化碳(CCl4)诱导的小鼠急性肝损伤模型,检测羟基积雪草苷(3、10、30 mg/kg)对血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)以及肝组织一氧化氮(NO)的影响;Western-blotting检测肝组织中诱导型一氧化氮合成酶(iNOS)、环氧合酶-2(COX-2)蛋白的表达,并取部分肝组织进行HE染色,观察病理学改变。结果羟基积雪草苷明显降低急性肝损伤小鼠血清中ALT、AST活性及肝组织中NO的量,抑制肝组织中iNOS、COX-2蛋白的表达,病理切片显示羟基积雪草苷组病变程度远较模型组为轻。结论羟基积雪草苷具有一定的肝保护作用,其机制可能与其抗炎作用有关。

Objective To discuss the protective effect and mechanism of madecassoside on mice with acute liver injury induced by CCl4. Methods Mice models of acute liver injury were provided with madecassoside at different doses （3, 10, and 30 mg/kg） separately. Serum function indexes including alanine aminotransferase （ALT） and aspartate aminotransferase （AST）, and contents of NO in liver tissue were measured according to the instruction of test kits. Western blotting was used for the analysis of protein expression of induced nitric oxide synthase （iNOS） and cyclooxygenase 2 （COX-2） in liver tissue. Some liver tissues were dyed with HE and histopathological changes in liver were observed under light microscope. Results The levels of ALT and AST in serum and the content of NO in liver tissue were reduced significantly by madecassoside in mice with acute liver injury. Meanwhile, the protein expression of iNOS and COX-2 in liver tissue was also inhibited by madecassoside with histopathological changes of liver more improved than that in model group. Conclusion Madecassoside is effective in amelioration of CCl4-induced hepatotoxicity. The effect may be due to its antinflammatory activity associated with less increase in hepatic iNOS and COX-2 protein content.