小叶黑柴胡药材的HPLC指纹图谱研究

HPLC Fingerprint of Bupleurum smithii var. parvifolia

目的建立小叶黑柴胡药材的HPLC指纹图谱。方法以Kromasil C18为色谱柱，流动相为乙腈-水梯度洗脱，体积流量1．0mL／min，检测波长260nm。结果建立了小叶黑柴胡药材的HPLC指纹图谱，确定了14个共有峰，共有峰相对保留时间的RSD为0．02％～1．49％，相对峰面积的RSD为6．12％～58．75％，10批样品的相似度均大于0．9。结论本方法简单可靠，可用于小叶黑柴胡药材的质量控制。

Objective To establish the HPLC fingerprint of Bupleurum smithii var. parvifolia. Methods Kromasil C18 column was used, with acetonitrile and water as the mobile phase in gradient mode, the flow rate was 1.0 mL/min, and the detection wavelength was 260 nm. Results The HPLC fingerprint of B. smithii var. parvifolia was established; There were 14 common peaks in the fingerprint of ten samples. The RSD of the relative relention time of the common peaks was 0. 02%-1. 49% and the RSD of the relative peak area was 6.12%-58.75%. The similarity of ten batches of samples was over 0. 9. Conclusion The method is simple, reliable, and can be used for the quality control of B. smithii var. parvifolia.