草分枝杆菌对SPCA/Ⅰ人肺腺癌细胞株诱导凋亡作用的研究

Study the effects of Mycobacterium Phlei on inducing apoptosis in SPCA/Ⅰ human lung adenocarcinoma cancer cells

目的:探讨草分子杆菌(Mycobacterium Phlei)对SPCA/Ⅰ人肺腺癌细胞株的抗增殖效应及诱导细胞凋亡的作用。方法:将SPCA/Ⅰ人肺腺癌细胞株培养传代,分别加入不同浓度的草分子杆菌,作用36h。分别以酶标仪检测细胞抑制率,FACSAria流式细胞仪检测细胞凋亡率,倒置显微镜观察凋亡细胞形态。结果:不同浓度的草分子杆菌体外对人肺腺癌细胞株SPCA/Ⅰ均有直接的抑制作用,其抑制率与所用药物浓度呈正相关。草分子杆菌体外对人肺腺癌细胞株SPCA/Ⅰ有诱导凋亡的作用,细胞凋亡率明显增加。倒置显微镜下可见细胞由原来贴壁生长而逐渐脱落、外形变圆、体积缩小、折光性增强,核染色质凝集,细胞裂解。结论:草分子杆菌能通过诱导细胞凋亡,直接抑制人肺腺癌细胞株SPCA/Ⅰ增殖。

Objective：To study the effects of Mycobacterium Phlei on cell apoptosis in SPCA/I human lung cancer cells. Methods： The Cells were cultured in RPMI 1640 together with Mycobacterium Phlei at different concentrations for 36 hour. The changes of apoptosis of SPCA/I human lung adenocarcinoma cells induced by Mycobacterium Phlei were detected by Annexin V： FITC Apoptosis detection kit,FACSAria flow cytometry and the inverted microscope. Results：The growth inhibitory rate had the positive correlations with concentrations of Mycobacterium Phlei on SPCA/I human lung adenocarcinoma cells. Mycobacterium Phlei had significant effects on cell apoptosis in SPCA/I human lung cancer cells. Typical apoptotic cells were seen whose bodies became smaller and rounder. Conclusion：Mycobacterium Phlei can induce apoptosis on SPCA/I human lung adenocarcinoma cells and inhibit the tumor cell proliferation.