河南省婴幼儿轮状病毒腹泻病原研究

Surveillance Result of Rotavirus of Babyhood Acute Diarrhea in Henan

目的了解河南省5岁以下儿童轮状病毒发病率，实验室检出率和病毒的基因分型。方法收集2005年10月-2006年10月两个监测点5岁以下儿童腹泻粪便标本585例，用酶联免疫吸附试验（ELISA）检测腹泻患儿粪便中的轮状病毒，然后用逆转录-聚合酶链反应（RT—PCR）方法对粪便中提取的轮状病毒RNA做基因分型。结果585份腹泻患儿粪便标本中检测出轮状病毒304份，全年轮状病毒阳性率51．97％。在304份ELISA阳性的标本中，随机取103份标本用RT—PCR法进行轮状病毒基因G．P分型，分型率为65．05％，其中P[8]G3为主要流行型，占所有分型的41．75％。结论轮状病毒腹泻仍然是河南省婴幼儿腹泻的主要病原体，但轮状病毒主要流行的血清型已由1990～1997年间监测的G1型转变为目前的G3型。

Objective To learn incidence ,positive specimens detected rate and genes serotyping of Rotavirus among children under 5 years of age in Henan. Methods 585 cases fecal specimens were collected in two survillance site from October 2005 to October 2006 of two survillance site in Henan. Rotavirus were detected by enzyme linked immunosorbent assay （ELISA）,and serotyping were determined by reverse transcription-polymerase chain reaction （RT-PCR）. Results During the one year of survillance,rotavirus were detected out 304 （51.97 % ） from 585 specimens. Among 103 specimens with G, P serotyping, the serotyping rate was 65.05% and the predominant strain was serotype P[8]G3 （41.75%）. Conclusion Rotavirus diarrhea still is the major cause of babyhood acute diarrhea in Henan,but epidemic seroty has changed for G1 1990-1997 to G3 present.