摘要
目的:建立HPLC法测定素清丸中的盐酸小檗碱含量。方法:采用Phenomenex C18 Gemini(250×4.6mm,5u)色谱柱,柱温为室温,以甲醇-0.2%磷酸溶液(45:55)为流动相;流速为1.0mL·min-1;检测波长:265nm。结果:盐酸小檗碱进样量0.215μg～1.075μg范围内与峰面积呈良好线性关系(r=0.9999),平均回收率为99.09%,RSD=1.3%(n=5)。结论:方法简便,结果准确,可作为本品质量控制的有效方法。
Objective：To establish a HPLC method for the quantitative determination of Berberine in Su Qing Pills. Methods： The determination was performed on Phenomenex C18 column with the mobile phase consisted of methanol--0.2 % phosphoric acid solution （45：55） at ambient temperature. The flow rate was 1.0ml·min^-1. The detection wave length was set at 265nm. Results： The method showed a good linear relationship within the range of Berberine was 0. 215μg-1. 07μg （r=0. 9999,n=5）. The average recovery rate was 99.09 % with RSD= 1.3 % （n= 5）. Conclusion： The methods is simple and accurate and could be used for quality control of Su Qing Pills.
出处
《内蒙古中医药》
2008年第9期22-23,共2页
Inner Mongolia Journal of Traditional Chinese Medicine
