药物建立大鼠室壁瘤模型及心功能与结构变化的评价

Rat model of ventricular aneurysm with isoproterenol and the changes of heart function and structure

目的探讨异丙肾上腺素建立大鼠室壁瘤模型的可行性及评价室壁瘤动物心功能与结构的变化。方法100只SD大鼠于腹股沟皮下注射异丙肾上腺素,250mg/kg,连续2d,饲养4周。结果79只成活的大鼠有49只发生室壁瘤,模型成功率为61.0%。血流动力学检测示室壁瘤大鼠左室压力最大上升速率(+dp/dt)下降和左室压力最大下降速率(-dp/dt)增加,P<0.001。超声检查示:注射药物后4周大鼠左室收缩和舒张末期内径(LVESD、LVEDD)增大,收缩和舒张末期容积(LVESV、LVEDV)变大,收缩功能(EF、FS%)降低,P<0.001;与无室壁瘤组相比,室壁瘤大鼠的LVESD和LVESV增大,EF和FS%降低,两者差异有统计学意义,P<0.05。注射药物前和注射药物后24h,血清肌酸激酶同工酶(creatine kinase-MB,CKMB)的浓度和血清肌钙蛋白T(cardiac troponin T,cTnT)的浓度明显升高,P<0.001。结论应用异丙肾上腺素建立大鼠室壁瘤模型是较为方便、可行的方法。

[Objective] To explore the feasibility of a rat model of ventricular aneurysm with isoproterenol and evaluate the changes of hear structure and function. [Methods] 100 SD rats were administered with a subcutaneous dose of isoproterenol at 250 mg/kg for 2 consecutive days. They were raised for 4 weeks. [Results] 49 of 79 rats underwent successful induction of aneurysm. After 4 weeks, regional wall motion abnormalities and cardiac dilatation were found by two dimensional eehoeardiography. As compared with before administration, left ventrieular and diastohc diameter （LVEDD）, left ventrieular end systolic diameter （LVESD）, left ventrieular end diastolic volume （LVEDV）, left ventricular end systolic volume （LVESV） increased, P 〈0.001. ejection factors （EF）, fractional shortening ratio （FS%） decreased, P〈0.05. The rats with aneurysms have larger LVESD, LVESV and more aggravated EF, FS% than those of without aneurysms, P 〈0.05. The absolute value of maximal rate of pressure rise （＋dp/dt） and the maximal rate of pressure fall （-dp/dt） was significantly deteriorated in the rats with aneurysms, P 〈0.001. Otherwise, the levels of ereatine kinase-MB （CKMB） and cardiac troponin T （eTnT） were increased after administration, P 〈 0.001. [Conclusion] It is feasible and convenient to contract a rat model of ventrieular aneurysm with isoproterenol, and the approach has a relatively high induction rate.