探讨CA19-9、CEA、COX-2、EGFR及VEGF检测在大肠癌患者诊治中的意义

Analysis significance of CA19-9,CEA,COX-2,EGFR and VEGF in patients with colorectal cancer

目的探讨CA19-9、CEA、COX-2、EGFR及VEGF检测在大肠癌患者中的意义。方法62例大肠癌患者,ELISA法检测大肠癌患者血清中CA19-9及CEA含量,免疫组化染色检测大肠癌组织中COX-2、EGFR及VEGF表达情况,另以10个正常人的血清及大肠组织为对照。结果62例患者中,CA19-9与CEA的阳性表达率分别为40.3%和50%,随Dukes分期越高,阳性率越高,差异有显著性。EGFR与VEGF的阳性表达率为48.4%和54.8%,与肿瘤分期、淋巴结转移及肿瘤大小成正相关,且他们之间的表达也具有相关性;COX-2的阳性表达率为58.1%,与大肠癌的Dukes'分期、淋巴转移、肿瘤的大小成正相关。结论这5个项目是互补性标志物,联合检测可提高大肠癌阳性检出率,VEGF和EGFR的表达具有相关性;检测这些指标不仅可以判断预后,而且可以指导治疗。

[Objective] To explore the significance of CA19-9, CEA, COX-2, EGFR and VEGF in patients with eoloreetal cancer. [Methods] Detect the content of CA19-9 and CEA in serum of 62 cases patients with coloreetal cancer by means of ELISA. Detect the expression of COX-2, EGFR and VEGF under Immunohistochemieal staining. Reference the same item in 10 cases normal. [Results] In 62 cases, the Positive rate of CA19-9 and CEA were 40.3% and 50% respectively, and have relationship to their Dukes＂ staging. The positive rate of EGFR and VEGF were 48.4% and 54.8% respectively, they have positive correlation with stage, metastasis and size of tumor, and they also have correlation with each other. The Positive rate of COX-2 was 58.1%, they have positive eorrelation with stage, metastasis and size of tumor. [Conclusion] The five index are complementary, conjoined detection can increase positive rate of colorectal cancer, EGFR and VEGF have correlation with each other. Detection of these index can not only judge prognosis, but guide the treatment.