医用电解质溶液作添加液制备汇集少白细胞血小板

Preparation of leukoreduced platelet concentrates from pooled buffy coats in a mixture of solutions for medical use

目的采用医用电解质溶液作添加液(PAS),建立1种白膜法制备添加液汇集少白细胞血小板(PAS汇集BC-PCs)的方法。方法从400ml全血中分离白膜层,容量35—40ml,放(22±2)℃静置过夜,将ABO同型的6袋白膜汇集,加220g添加液(90%复方电解质注射液、8%ACD-A血液保养液和2%含量为50g/L的碳酸氢钠注射液的混合液)稀释白膜,汇集后的白膜在(22±2)℃中,以300×g离心10min,将上层的富含血小板悬液再经白细胞滤除器过滤去除白细胞,并转移到血小板保存袋内,即制备成1个成人治疗量的PAS汇集BC-PCs,制备过程在一个特制的密闭系统内完成。结果共制备30个成人治疗量的PAS汇集BC-PCs,其容量为(270±32)ml、血小板含量为(2.96±0.31)×1011、WBC混入量为(1.3±0.2)×106、RBC混入量为(5.8±1.1)×109、CD62P表达率为(22.5±10.6)%。保存8d后的pH为7.14±0.04、低渗休克反应率(HSR)为(54.0±8.2)%、CD62P表达率为(45.7±13.8)%。结论由复方电解质注射液、ACD-A保养液和碳酸氢钠注射液的混合液为添加液汇集血小板的方法可行。

Objective To develop a method to produce leukoreduced platelet concentrates （LR-PCs） from pooled buffy coats in additive solution （ a mixture of solutions for medical use ）. Methods LR-PCs were made from 6 pooled buff）, coats （12 whole-blood units） and 220g additive solution, including 90% multiple electrolytes injection solution, 8% ACDA and 2% 50g/L NaHCO3 injection solution. After centrifugation, the PCs were leukoreduced with a filter and stored in a 600ml platelet storage bag. LR-PCs were prepared in a closed system. Results Routinely produced LR-PCs （ n = 30 ） contained {2.96 ±0. 31 ） × 10^11 platelets with a volume of （270 ± 32）ml. The WBC and RBC count were （ 1.3±0. 2） × 10^6 and （5.8 ± 1.1 ） × 10^9 per unit, respectively. The CD62P expression was （22.5 ± 10. 6） %. After 8-day storage, the in vitro quality of LR-PCs, including pH, hypotonic shock response （HSR） and CD62P expression were 7.14 ± 0. 04, （54. 0± 8.2） % and （ 45.7 ±13.8 ） %, respectively. Conclusion In terms of the in vitro quality of LR - PCs, the method of preparing LR-PCs from pooled bully coats in mixing solutions is feasible.