HPLC法测定阿昔洛韦片中阿昔洛韦的含量

Content determination of acyclovir in Acyclovir Tablets by HPLC

目的:建立阿昔洛韦片中阿昔洛韦的含量测定方法。方法:采用高效液相色谱法,以十八烷基硅烷键合硅胶为填充剂,色谱柱为HypersilC18柱(4.6mm×200mm,5μm);流动相为甲醇-水(10∶90);流速1.0ml/min;检测波长254nm;柱温:室温;进样量20μl。按外标法以峰面积计算阿昔洛韦含量(以标示量的百分含量表示)。结果:阿昔洛韦浓度在5～30μg/ml范围内与峰面积呈良好的线性关系;平均回收率为100.00%,RSD=0.22%;三批样品的平均含量是98.9%,RSD=0.20%。结论:本方法专属性强、灵敏度高、重现性好,可用于阿昔洛韦的含量测定。

Objective：To establish a method for the content determination of acyclovir. Methods：HPLC method was adopted with Hypersil Cls column（4.6 mm×200 mm,5μm）, the mobile phase was methanol-water （10：90） and flow rate was 1.0 ml/min.The detector wavelength was 254 nm. External standard method was used for the content calcaulation. Results： Acyclovir had a good linearity between 5~30 μg/ml. The average recovery was 100.00% with RSD of 0.22%. The average content of the three samples was 98.9% with RSD of 0.20%. Conclusion：The method has good specifity,sensitivity and reproducibility, can be used for the content determination of acyclovir.