猕猴桃高效离体再生体系的研究

Study on High Efficient Reproduction System of Kiwifruit in vitro Culture

为建立猕猴桃高效离体再生体系,试验以猕猴桃离体茎尖和茎段作外植体,在初代培养诱导试管苗成功的基础上,分别以MS和1/2MS为基本培养基,附加不同的激素组合,研究不同培养基对猕猴桃叶片和叶柄芽诱导的影响和对猕猴桃生根的影响。试验结果表明,在MS+1.0mg/L6-BA+0.1mg/LNAA的培养基中培养,初代苗诱导成功、生长良好;在MS+2.0mg/L6-BA+0.1mg/LNAA的培养基中,平均每个叶片、叶柄外植体芽诱导数分别为1.38和5.13;在1/2MS+0.9mg/LIBA和1/2MS+0.6mg/LIBA的培养基诱导生根效果好,生根数、根长统计均显著高于其他处理;温室、自然光照条件下封闭瓶口炼苗1周后,揭开封口膜再炼苗1周,以消过毒的珍珠岩和草炭土(3:7)为移栽基质,成活率达到80%。试验结果为猕猴桃转基因育种及优良品种快速繁殖研究提供了依据。

The stem segment and apex of kiwifruit （Actinidia deliciousa） were taken as explants and cultured to establish high regeneration system in vitro. The effect of different hormonal combinations in the MS and 1/2 MS medium to the rate of bud induced from leaf and petiole and rooting were studied after having gained initial in vitro plantlet successfully. The result showed that the initial plantlet grew well in the basic MS medium added 1.0 mg/L 6-BA and 0.1 mg/L NAA. In the medium of MS ＋2.0 mg/L 6-BA ＋0.1 mg/L NAA the rates of induction budding from leaf and petiole were 1.38, 5.13 respectively. The 1/2 MS medium added 0.9 mg/L and 0.6 mg/L IBA were effective to rooting, the number and length of root was higher than other treatments markedly. The survive rate of plantlet transplanted into the substrate composed of 30 % perlite and 70 % peat-moss was 80 % after the plantlet was trained under the condition of room temperature and natural light covering and uncovering one week respectively. The achievement of the study has laid a foundation for transgenic breeding and rapid propagation in kiwifruit.