古蜡梅的组织培养和试管苗的移栽及栽培技术

Tissue Culture of Hundred Year Old-Chimonanthus praecox and Transplanting and Cultivating of Tube-plants

以提高古蜡梅的组织培养和试管苗移栽及栽培效果为目的,用嘉定钱门塘的古蜡梅为起始材料,进行了无菌苗诱导、快速繁殖、生根和移栽试验。结果表明,1/2MS作为基本培养基明显优于Read、WPM、N6;诱导培养基中添加2.0mg/L2iP、0.2mg/LIAA、0.5mg/LGA3和1.5-5.0mg/LPVP可提高古蜡梅的繁殖系数和抑制外植体褐化;适当降低快繁培养基中的激素浓度可以防止玻璃苗的发生;添加0.5mg/LIBA和0.05mg/LNAA可使古蜡梅组培苗的生根率在75%左右。试管苗移栽前进行1-2d的炼苗和移栽后2周内保湿防晒可以提高成活率;抑制杂草可以促进试管苗健壮生长;控制地下水位可以提高试管苗的根系活力。

For the purposes of enhancing the effects of tissue culturing, tube-plantlets transplanting and cultivating, the annual branches of hundred-years-old Chimonanthus praecox were used as donor materials. Shoot induction, rapid propagation, root induction and cultivation were investigated, and the results showed that the 1/2MS （1/2 of macroelements） as basic media was superior to Read、WPM and N6, the propagation coeifficient was increased and the frequency of explants browning was decreased by supplementing with 2.0mg/L 2iP, 0.2mg/L IAA, 0.5 mg/L GA3 and 1.5-5.0mg/L PVP to the inducing media, the number of vitreous buds were decreased by reducing hormone concentrations of the propagation media, the frequency of rooting was 75% in media contained 0.5mg/L IBA and 0.05mg/L NAA. The transplanting survival rate of tube-plantlets was improved by hardening the plantlets for 1-2 days and maintaining humidity for 14 days. The growth of tube- plantlets was promoted by holding weeds, and root-vigor was increased by controlling the level of groundwater.