凉润通络方及其拆方对糖尿病大鼠胃肠MOT、CCK、SS的影响

Effect of Liangruntongluo Recipe and its Modified Formula on Gastrointestinal MOT. CCK. SS of Rats with Diabetes

目的探讨凉润通络方及其拆方对糖尿病大鼠胃肠运动功能改善的机制。方法雄性Wistar大鼠，注射链脲佐菌素（STZ）制作糖尿病大鼠模型。造模成功后，不予降糖药控制血糖，正常饲养18周后，大鼠分别予温水、凉润通络中药、单纯通络中药、单纯滋润中药、单纯清凉中药、西沙必利灌胃，6周后检测血浆中胃动素（MOT）、胆囊收缩素（CCK）、生长抑素（SS）。结果24周时糖尿病大鼠存在胃肠激素的紊乱，凉润通络方影响血液中胃肠激素的分泌和释放，作用靶点较宽，优于其拆方和西沙必利。结论凉润通络方可改善糖尿病大鼠胃肠功能障碍。

Objective To study the mechanism of Liangmntongluo Recipe （Chinese medicines with functions of cooling, nourishing and dredging collaterals） and its modified formula in improving gastrointestinal function of diabete smellitus （DM） rats. Methods Male Wistar rats were used. Streptozotocin （STZ） was injected to rats to produce diabetic rat models. No Hypoglycemic drugs were administered to these rats to reduce blood glucose. After 18 weeks, warm water, Liangruntongluo Recipe, Chinese medicines with function of nourishing, Chinese medicines with functions of cooling, and Cisapride were administered to the model rats. Detect the contents of plasma motilin （MOT）, cholecystokinin （CCK）, and somatostatin （SS） after 6 weeks. Results By affecting the secretion of gut hormone and having wide range of target, Liangruntongluo Recipe could regulate the disorder of gut hormone. The function of Liangruntongluo Recipe was better than its modified formula and cisapride. Condusion Liangruntongluo Recipe can improve gasurointestinal dysfunction of DM rats.