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2型猪链球菌假想转运蛋白89k/pTP与毒力的相关分析 被引量：1

Analysis of the Correlation Between the Putative Transporter 89k/pTP and Virulence for Streptococcus suis Serotype 2
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摘要 目的:构建2型猪链球菌假想转运蛋白89k/pTP的基因插入失活突变体并进行毒力相关分析。方法:首先将同源臂片段克隆到pSET4s质粒上,构建插入失活载体pSET4s-pTP,并通过对单交换的筛选获得89k/pTP的基因插入失活突变体,再用小鼠腹腔感染模型对突变株和野生株的毒力进行比较。结果:获得了89k/pTP的基因插入失活突变体,并发现其毒力与野生型相比明显下降。结论:2型猪链球菌假想转运蛋白89k/pTP与其毒力有关,其作用和机制值得进一步分析。 Objective： To construct insertional inactivation mutant of the putative transporter 89k/pTP of Streptococcus suis serotype 2 and to give a correlation analysis for its virulence. Methods： The homologous ann was cloned into suicide vector pSET4s to build inactivation plasmid pSET4s-pTP, and then the insertional inactivation mutant 89k/pTP which was aquired by verifing the single-exchange clones was obtained. The comparison of the mutant and wild type was analysed by the mice infection model. Results： The insertional inactivation mutant of 89k/pTP was constructed successfully and its virulence attenuated significantly than wild type. Conclusion： The putative transporter 89k/pTP contributes to the virulence of S.suis serotype 2, and the role and mechanism of this protein is valuable for futher analysis.
作者 袁媛 路玲玲 李文君 郑玉玲 姜永强
机构地区 军事医学科学院微生物流行病学研究所
出处 《生物技术通讯》 CAS 2008年第5期685-688,共4页 Letters in Biotechnology
基金 国家自然科学基金(30600023)
关键词 2型猪链球菌 假想转运蛋白 毒力 插入失活突变体 Streptococcus.suis Serotype 2 putative transporter virulence insertional inactivation mutant
分类号 Q78 [生物学—分子生物学]
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参考文献16

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同被引文献22

  • 1Aguilera L, Ferreira E, Gimenez R, et al. Secretion of the housekeeping protein glyceraldehyde- 3-phosphate dehydrogenase by the LEE-encoded type III secretion system in enteropathogenic Escherichia co/i[J]. The International Journal of Biochemistry and Cell Biology, 2012, 44(6): 955-962.
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  • 6Li M, Shen X, Van J, et al. Gl-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2[J). Molecular Microbiology, 2011, 79(6): 1670-1683.
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  • 8Stephenson K, Hoch JA. Two-component and phosphorelay signal-transduction systems as therapeutic targets[J]. Current Opinion in Pharmacology, 2002, 2(5): 507-512.
  • 9Li M, Wang CJ, Feng YJ, et al. SalKfSaIR, a two-component signal transduction system, is essential for full virulence of highly invasive Streptococcus suis serotype 2(1). PLoS One, 2008, 3(5): e2080.
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生物技术通讯
2008年 第5期

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