Aβ_(1-40)诱导皮层神经元凋亡和人参二醇的保护作用

Cortical neuron apoptosis induced by β-amyloid peptide and protective effect of panoxadiol in mice

目的:研究β-淀粉样肽(Aβ1-40)对原代培养的小鼠大脑皮层神经细胞凋亡的诱导和人参二醇(panoxadiol)的保护作用。方法:通过形态学观察,MTT法检测,DNA琼脂糖凝胶电泳,Western-blot等方法研究Aβ1-40对皮层神经元的损伤及人参二醇(由浙江省中医院提供)的保护作用。结果:原代培养的小鼠大脑神经细胞经12μmol/LAβ1-40作用48h后,Aβ1-40损伤组神经细胞出现明显的凋亡特征,OD值降低,DNA电泳出现DNA片段,凋亡细胞数目增多。Western-blot显示Aβ1-40损伤组bcl-2表达下降(P<0.05),而40mg/L人参二醇预处理24h后可明显改变上述凋亡特征。结论:40mg/L人参二醇可减缓12μmol/LAβ1-40诱导培养皮层神经元凋亡,对凋亡细胞有一定的保护作用。

Objective： To investigate the apoptosis of cortical neurons induced by β-amyloid peptide （Aβ1-40） and the protective effect of panoxadiol. Methods： The Aβ1-40 induced damage of primarily cultured mouse cortical neurons was examined with morphological observation,MTT assay,DNA agarose gel electrophoresis and Western-blot. Results： After 48 h treated with 12 μmol/L Aβ1-40,the cortical neurons showed apoptotic characteristics：including decreased OD570 value in MTT assay,DNA cleavage fragment in electrophoresis and increased apoptotic cells. Western-blot showed that the expression of bcl-2 reduced significantly （P〈0.05）. Cell apoptosis was significantly attenuated in 40 mg/L panoxadiol treated group. Conclusion： Panoxadiol can protect cultured cortical neurons from apoptosis induced by Aβ1-40 in mice.