期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

TGF-β减轻异基因骨髓移植小鼠急性移植物抗宿主病 被引量：1

Transforming Growth Factor β Alleviates Acute Graft-versus-Host-Disease after Allogeneic Bone Marrow Transplantation in Murine Model
下载PDF
导出
摘要 为了探讨转化生长因子β(TGF-β)对小鼠异基因骨髓移植后急性移植物抗宿主病发生发展的影响,用C57BL/6小鼠作为供鼠,BABL/c小鼠为受鼠,建立小鼠同种异基因骨髓移植模型。BABL/c受鼠随机分为4组:空白对照组、单纯照射组、移植对照组和TGF-β实验组。TGF-β实验组于移植前2天至移植后7天每日皮下注射TGF-β1(1μg/kg)。结果显示:TGF-β实验组小鼠生存时间明显长于移植对照组(p<0.01),TGF-β实验组小鼠小肠、皮肤及肝脏GVHD病理改变明显轻于移植对照组。移植后7天TGF-β实验组小鼠血清IL-2浓度较空白对照组高,但远低于移植对照组;TGF-β实验组血清IL-10浓度明显高于移植对照组。结论:TGF-β能够减轻或抑制小鼠allo-BMT后致死性的aGVHD发生,提高移植后存活率。TGF-β可能使Th1细胞向Th2细胞偏移,抑制IL-2等Th1类细胞因子的分泌,同时促进了Th2类细胞因子IL-10的表达,降低aGVHD的发生。 This study was purposed to investigate the effects and mechanism of transforming growth factor beta （TGF-β） on acute graft-versus-host disease （aGVHD） after allogeneic bone marrow transplantation （allo-BMT）. The recipients were male BABL/c mice, while the donors were male C57BL/6 mice. The routine model of aGVHD had been established by allo-BMT with donor derived T cells. Experiment was divided into four groups： control group, radiation control group, transplantation control group and TGF-β treated group. Mice in TGF-β treated group were daily subcutaneaesly injected TGF-β1 （1 μg/kg） in two days before transplantation until seven days after it. The results showed that the survival time of mice in TGF-β treated group was significantly longer than that in transplantation control group, and the aGVHD pathological changes in TGF-β treated group were milder than that in transplantation control group. At seven days after transplantation, the level of IL-2 in TGF-β treated group was significantly higher than that in control group, but significantly lower than that in transplantation control group. The level of IL-10 in TGF-β treated group was significantly higher than that in transplantation control group, but the level of IL-10 in transplantation control group was significantly lower than that in other groups. It is concluded that TGF-β may alleviate or suppress lethal aGVHD, and elevate the survival rate after allo-BMT in murine model. Accommodatin of the Thl and Th2 cytokine levels is the possible mechanism of TGF-β preventing lethal aGVHD.
作者 王旖旎 冯翠翠 刘锦丽 李芳 付丽 王昭
机构地区 首都医科大学附属北京友谊医院血液科
出处 《中国实验血液学杂志》 CAS CSCD 2008年第5期1135-1139,共5页 Journal of Experimental Hematology
关键词 转化生长因子Β 异基因骨髓移植 急性移植物抗宿主病 细胞因子 TGF-β alIo-BMT aGVHD cytokine
分类号 R547.7 [医药卫生—心血管疾病]
  • 相关文献

参考文献9

  • 1Ringden O, Labopin M, Gorin NC, et al. Treatment with granulocyte colony- stimulationg factor after allogeneic bone marrow transplantation for acute leukemia increases the risk of graftversus- host disease and death: a study from the acute leukemia working party of the european group for blood and marrow transplantation. J Clin Oncology, 2004; 22:416-423
  • 2King WJ, Comer RM, Hudde T, et al. Cytokine and chemokine expression kinetics after corneal transplantation. Transplantation, 2000; 70:1225-1233
  • 3Hattori H, Matsuzaki A, Suminoe A, et al. Polymorphisms of transforming growth factor-betal and transforming growth factorbetal type Ⅱ receptor genes are associated with acute graft-versushost disease in children with HLA-matched sibling bone marrow transplantation. Bone Marrow Transplant, 2002; 30:665-671
  • 4黄雅静,孙琪云,刘丽辉,胡恺勋,范传波,边莉,郭梅,艾辉胜.猕猴单倍体相合外周血造血干细胞移植后多种细胞因子mRNA表达的动态分析[J].中国实验血液学杂志,2006,14(3):571-576. 被引量：1
  • 5Hill GR, Ferrara JL. The primacy of the gastrointestinal tract as a target organ of acute graft -verus -host disease ; rationale for the use of cytokine shields in allogeneic bone marrow transplantation. Blood, 2000 ; 95 : 2754 - 2759
  • 6Zeller JC, Panoskaltsis-Mortari A, Murphy WJ, et al. Induction of CD4^+ T cell alloantigen- specific hyporesponsiveness by IL-10 and TGF-β1. J Immunol, 1999; 163:3684-3691
  • 7Hodge GL, Hodge SJ, Nairn J, et al. Poststorage leuko-depleted plasma inhibits T-cell proliferation and Thl response in vitro: characterization of TGF beta-1 as an important immunomodulatory componentin stored blood. Transplantation, 2005; 80: 95- 101
  • 8Fortunel NO, Hatzfeld A, Hatzfeld JA. Transforming growth factor-beta: pleiotropic role in the regulation of hematopoiesis. Blood, 2000 ; 96 : 2022 - 2036
  • 9Kale VP. Differential activation of MAPK signalling pathways by TGF-betal forms the molecular mechanism behind its dosedependent bidirectional effects on hematopoiesis, Stern Cells Dev, 2004; 13:27-38

二级参考文献22

  • 1Ferrara JL,Levy R,Chao NJ.Pathophysiologic mechanisms of acute graft -vs.-host disease.Biol Blood Marrow Transplant,1995 ;5:347 -356
  • 2Ferrara JL.The cytokine modulation of acute graft-versus-host disease.Bone Marrow Transplant,1998 ;21 (Suppl 3):S13-S15
  • 3Antin JH,Fenera JL.Cytokine dysregulation and acute graft-versushost disease.Blood,1992 ;80:2964-2968
  • 4Min CK,Lee WY,Min DJ,et al.The kinetics lf circulating cytokines including IL-6,TNF-γ,IL-8 and IL-10 following allogeneic hematopoietic stem cell transplantation.Bone Marrow Transplant,2001 ;28:935 -940
  • 5Holler E,Roncarolo MG,Hintermeier-Knabe R,et al.Prognostic significance of increased IL-10 production in patients prior to allogeneic bone marrow transplantation.Bone Marrow Transplant,2000;25:237-241
  • 6Sakata n,Yasui M,Okamura T,et al.Kinetics of plasma cytokines after hematopoietic stem cell transplantation from unrelated donors:the ratio of plasma IL-10/sTNFR level as a potential prognostic marker in severe acute graft-versus-host disease.Bone Marrow Transplant,2001; 27:1153-1161
  • 7Miura Y,Thoburn CJ,Bright EC,et al.Cytokine and chemokine profiles in autologous graft-versus-host disease (GVHD):interleukin10 and interferon gamma may be critical mediators for the development of autologous GVHD.Blood,2002; 100:2650-2658
  • 8Takatsuka H,Takemoto Y,Okamoto T,et al.Predicting the severity of graft-versus-host disease from interleukin-10 levels after bone marrow transplantation.Bone Marrow Transplant,1999; 24:1005-1007
  • 9Ferra C,de-Sanjose S,Gallardo D,et al.IL-6 and IL-8 levels in plasma during hematopoietic progenitor transplantation.Hematologica,1998 ;83:1082-1087
  • 10Steffen M,Durken M,Pichlmeier U,et al.Serum interleukin-6 levels during bone marrow transplantation:impact on transplant-related toxicity and engraftment.Bone Marrow Transplant,1996;18:301 -307

同被引文献10

  • 1Yoneda O, Imai T, Goda S, et al. Fractalkine-mediated endothelial cell injury by NK cells. J Immunol,2000 ; 164 (8) :4055 -4062.
  • 2Le Bas-Bernardet S, Hourmant M, Coupel S, et al. Non-HLA-type endothelial cell reactive alloantibodies in pre-transplant sera of kidney recipients trigger apoptosis. Am J Transplant, 2003;3 (2) :167 - 177.
  • 3Denton MD, Davis SF, Baum MA, et al. The role of the graft endothelium in transplant rejection:evidence that endothelial activation may serve as a clinical marker for the development of chronic rejection. Pediatr Transplant, 2000 ; 4 (4) :252 - 260.
  • 4Cook Mills JM. VCAM-1 signals during lymphocyte emigration: role of reactive oxygen species. Mol Immunol, 2002 ; 39 ( 9 ) :499 - 508.
  • 5Markovic S, Raab M, Daxecker H, et al. In vitro effects of cyclosporine A on the expression of adhesion molecules on human umbilical vein endothelial cells. Clin Chim Acta, 2002; 316 (1 -2 ) :25 -31.
  • 6Hjortoe GM, Petersen LC, Albrektsen T, et al. Tissue factor-factor Via-specific up regulation of IL-8 expression in MDA-MB3-231 cells is mediated by PAR-2 and results in increased cell migration. Blood, 2004; 103(8) :3029 -3037.
  • 7Markovic S, Raab M, Daxecker H ,et al. In vitro effects of cyelosporine A on the expression of adhesion molecules on human umbilical vein endothelial cells. Clin Chim Acta, 2002 ; 316 ( l - 2) :25 - 31.
  • 8Charreau B, Coupel S, Goret F, et al. Association of glucocorticoids and cyclosporine A or rapamycin prevents E-selectin and IL-8 expression during LPS- and TNFalpha-mediated endothelial cell activation. Transplantation, 2000 ; 69 (5) :945 - 953.
  • 9A. Naji, F. Deschaseaux E, Racadot C, et al. Induction of tissue factor expression on human umbilical vein endothelial cells by cell- specific HLA class I antibody: preliminary data. Transplantation Proceedings, 2005; 37: 2892- 2893.
  • 10陆轩,萧明第,尤文俊.豚鼠-大鼠异种心脏移植急性血管排斥反应期组织因子mRNA的表达[J].复旦学报（医学版）,2003,30(5):463-465. 被引量：1

引证文献1

二级引证文献2

中国实验血液学杂志
2008年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部