绿色荧光蛋白标记的报告系统优化K562细胞转染条件的研究

GFP Used as A Reporter System for Optimization of K562 Cell Transfection

本研究旨在提高基因转染人红白血病细胞株K562的转染效率,同时观察绿色荧光蛋白(GFP)作为报告系统用于转染条件优化的可行性。目的基因以GFP为报告系统,通过脂质体转染及电穿孔两种方式转染K562细胞,荧光显微镜下观察并计算转染效率。结果表明:电转染较脂质体转染K562细胞的效率高,电穿孔转染效率在10%左右,而脂质体转染效率小于1%。结论:电转染较脂质体转染K562细胞的效率高,GFP可作为报告系统优化K562细胞转染条件。

This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein （GFP） as a reporter system. Transfection efficiencies with different methods including electroporation and lipofectamine 2000 transfection, were observed and calculated under fluorescent microscopy by using GFP as a reporter system. The results showed that the transfection efficiency with electroporation（ 10% ） was higher than that with lipofectamine 2000 （ 1% ）. In conclusion, the electroporation is a more ideal method for introduction of foreign gene into K562 cells. GFP can be used as a reporter system for optimizing transfection of K562 cells.