摘要
Objective:To identify potential serum markers of hepatic carcinoma in rats through Surface-Enhanced Laser Desorption Ioniza- tion-Time of Flight-Mass Spectrometry(SELDI-TOF-MS)Technology.Methods:A rat model of hepatic carcinoma was established. The serum samples of hepatic carcinoma and normal rats were analyzed via SELDI-TOF-MS Technology.The changes of the serum protein fingerprint patterns were observed between the experimental group of hepatic carcinoma and the controls.The analysis was conducted by statistical software-Biomarker Wizard.Results:Fifty-six protein peaks in the serums were found.Within m/z 0-20 000, the protein peaks of m/z 1158,8 835 and 15 302 of hepatic carcinoma serums were obviously higher in the rat models compared with those in the controls(P<0.01).Conclusion:Three peaks were considered as potential biomarkers according to the serum protein fingerprint patterns of the hepatic carcinoma group and the control group.
Objective： To identify potential serum markers of hepatic carcinoma in rats through Surface-Enhanced Laser Desorption Ionization-Time of Flight-Mass Spectrometry（SELDI-TOF-MS） Technology. Methods： A rat model of hepatic carcinoma was established. The serum samples of hepatic carcinoma and normal rats were analyzed via SELDI-TOF-MS Technology. The changes of the serum protein fingerprint patterns were observed between the experimental group of hepatic carcinoma and the controls. The analysis was conducted by statistical software-Biomarker Wizard. Results： Fifty-six protein peaks in the serums were found. Within m/z 0-20 000, the protein peaks of rrdz 1158, 8 835 and 15 302 of hepatic carcinoma serums were obviously higher in the rat models compared with those in the controls（P 〈 0.01）. Conclusion： Three peaks were considered as potential biomarkers according to the serum protein fingerprint patterns of the hepatic carcinoma group and the control group.
基金
the Science Foundation of the Ministryof Health of China(No.wkj2004-2-12).
