CTN株疫苗对中国狂犬病病毒街毒代表株的保护性

Protection of Rabies Vaccine Prepared with CTN Strain Against Challenge with Representative Rabies Street Virus Strains in China

目的研究人用狂犬病病毒CTN株疫苗对中国狂犬病病毒街毒代表株的保护性,为评估该疫苗的适用性提供依据。方法用CTN株疫苗免疫昆明小鼠,用3株具有代表性的街毒株CQ92、HN06、J和标准攻击毒株CVS经脑内和肌肉攻击,以未免疫小鼠进行脑内和肌肉攻击为对照,观察CTN株疫苗的保护效果。结果原倍和5倍稀释的疫苗免疫的小鼠经3株街毒肌肉攻击后,均得到100%的保护,与对照组比较,差异均有极显著意义;经3株街毒脑内攻击后,原倍疫苗免疫的小鼠均得到100%的保护,5倍稀释的疫苗免疫的小鼠对3株街毒CQ92、HN06、J的保护率分别为85%、75%和77.8%,与对照组比较,差异均有极显著意义。结论CTN株疫苗总体上能有效预防我国目前狂犬病的流行。

Objective To study the protection of rabies vaccine prepared with CTN strain against challenge with representative rabies street virus strains in China and provide basis for evaluation of applicability of the vaccine. Methods Immunize KM mice with rabies vaccine prepared with CTN strain then challenge with 3 representative rabies street virus strains, i. e. CQ92, HN06 and J, by intracerebral and intramuscular routes separately. Evaluate the protective effect of vaccine on mice, using the unimmunized mice as control. Results All the protective rates of original and 1：5 diluted vaccine against the challenge i.m. with 5 street virus strains were 100%, which were significantly higher than those of control. The protective rates of original vaccine against the challenge i.c. with CQ92, HN06 and J strains were 100%, while those of 1：5 diluted vaccine were 85%, 75% and 77.8% respectively, which were still significantly higher than those of control. Conclusion At present, the rabies vaccine prepared with CTN strain may prevent the epidemic of rabies in China effectively in general.