一株海洋细菌MZ0306A1的16SrDNA扩增及序列分析

The 16S rDNA amplification and sequence analysis of a marine bacterium MZ0306A1

PCR技术应用于环境监测具有快速、灵敏、准确、简便、特异性强等特点,被广泛应用于水体、气体和土壤的环境污染监测中。将此方法应用于海洋细菌监测中,从浙江南部沿海表层海水中分离到一林海洋细菌MZ0306A1,利用细菌通用引物对其16S rDNA进行扩增。琼脂糖凝胶电泳检测目的DNA片段约为1.5 kb。测序后将所测得的序列在NCBI网站中进行相似性搜索,得到该细菌的结果与Halomonas variabilis strain DSM 3051的相似性最高,为97%,故命名为嗜盐单胞菌属。该细菌的16S rDNA序列已经提交GenBank,序列收录号为EU124745。

PCR technique used in environmental monitoring has the character of rapidity, accuracy, simplicity and a strong peculiarity, which can be widely used in water, air and soil environmental pollution monitoring. This technique was used in marine bacteria monitoring in this paper. A marine bacterium MZ0306A1 was isolated from the surface layer of seawater of the southern Zhejiang province seaboard. The 16S rDNA sequence was gained by PCR using the general 16S rDNA primers. The 1. 5% agarose electrophoresis showed the target fragment was about 1.5 kb. Neighbor-joining phylogenetic trees were drawn by compa- ring the results of 16S rDNA sequencing with sequences of described species in the BLAST server of the National Center for Biotechnology Information （NCBI）. The strain MZ0306A1 was 97% similar to the Halomonas variabilis strain DSM 3051, which was named as Halomonas sp. The 16S rDNA sequence of MZ0306A1 had been submitted tO the GenBank and the accession number was EU124745.