运用导流杂交基因芯片技术在重庆地区人乳头瘤病毒感染分型检测的研究

Application of flow - through hybrization and gene chipon human papillomavious subtype detection in Chongqing

目的探讨运用导流杂交基因芯片技术（HybriMax）在临床人乳头瘤病毒（HPV）感染分型检测中的运用，调查重庆地区宫颈病变患者HPV病毒分型情况进行。方法采用导流杂交基因芯片技术，对473例宫颈病变患者以病理学诊断为金标准对宫颈病变进行分组后，进行生殖道HPV感染分型检测。结果17种HPV亚型被检出，总检出率为63．0％（284／473），检出率较高的亚型为HPV16（23．7％、112／473）、HPV58（12．7％、60／473）、HPV53（7．4％、35／473）。HPV感染率随宫颈病变SPI、CINⅠ、CINⅡ、CINⅢ的变化而升高（x^2=77．06、P〈0．01）。结论重庆地区最常见的HPV型别是HPV16、58型。导流杂交基因芯片技术能够同时检测HR—HPV和LR—HPV，可判断多型感染；用于临床诊断和普查均为理想方法。

Objective To evaluate the application of flow- through rapid hybridization technique and gene chip （HybriMax） on human papillomavious （HPV） subtype in Chongqing. Methods Cervical tissue samples were taken under the colposcope form 473 females who had cervical lesion for pathological analysis. The predictive value of HybriMax in cervical abnormality was compared with pathological resultS, which were used as golden standard. Results 13 different subtypes were found and total HPV positive rate was 63.0% （284/473）Among the 17 different subtypes ,the higher positive rate HPV subtypes were HPV16 （ 23.7%, 112/473 ）, HPV58 （ 12.7% ,60/473 ）, HPV53（7.4% ,35/473）. The HPV infection rates were higher with the worse of cervical lesion（ X2 = 77. 06,P 〈 0. 01 ）. Conclusions The most frequent subtypes of HPV infection in Chongqing cervical lesion were HPV 16,58. HyhriMax was an effective method to detect HPV subtype in clinical.