哮喘患儿血清IL-12、IL-4与IgE水平的变化及意义

Research on change of levels of IL-12 ,IL-4 and total IgE in asthmatic children

目的研究哮喘患儿白细胞介素12（IL-12）、白细胞介素4（IL-4）与免疫球蛋白E（IgE）水平的变化。方法采用酶联免疫（ELISA）法测定不同时期支气管哮喘患儿血清IL-12、IL-4和IgE水平，并与健康对照组进行统计分析。结果哮喘急性发作组血清IL-12水平显著低于缓解组和对照组（P〈0．05），而IL-4和IgE水平却显著高于缓解期和对照组（P〈0．05）。IL-12与IgE呈负相关（r=-0．574，P〈0．05），而IL-4与IgE呈正相关（r=0．587，P〈0．05）。结论IL-12和IL-4参与支气管哮喘发病的整个过程，IL-12降低和IL-4升高是哮喘发病的重要因素。

Objective Objective Research on the change of levels of interleukin 12 （IL-12）, interleukin4 （IL-4） and immunoglobulin E（ Ig E）in asthmatic children. Methods 20 asthmatic children in attack stage and 20 asthmatic children in remission stage were as sample cases. The serum levels of IL-12 ,IL-4 and Ig E were detect with ELISA. Results IL-12 level of children in attack stage was obviously lower than those in remission stage and healthy children （ P 〈 0.05 ） ; IL-4 and Ig E level of children in attack stage was obviously higher than those in remission stage and healthy children （ P 〈 0. 05 ） ; IL-4 level of children in remission stage was obviously higher than healthy children （P 〈0.05） ;The level of IL-12 was in negative correlation with that of IgE in asthmatic children, while the level of IL-4 was in positive correlation with that of IgE in asthmatic children. Conclusions Asthmatic children have lower level of IL-12 and higher levels of IL-4 and IgE.