大鼠心内神经节雌激素受体和免疫因子白介素6的共存

The coexistence of estrogen receptor and interleukin-6 in the intra-cardiac ganglia of rats

目的：研究雌激素受体（ER）和免疫因子白介素6（IL-6）在心内神经节的表达,并证实它们共存的可能性。方法：免疫细胞化学双重标记技术。结果：切片上观察到3种细胞：①ER单标细胞,胞核呈棕褐色;②IL-6单标细胞,胞浆呈红色;③ER/IL-6双标细胞,胞核棕褐色,胞浆为红色。双标细胞占全部阳性标记细胞的20%~30%。结论：ER和IL-6可共存于同一大鼠心内神经节细胞,从而在细胞水平为神经免疫内分泌网络在心脏的存在提供了直接的形态学证据。

Objective：To investigate the expression of estrogen receptor（ER） and interleukin-6（IL-6） in the intra-cardiac ganglia of rats and to identify the coexistence of these two proteins in the intra-cardiac ganglia.Methods：The double labeling techniques of immunocytochemical staining were conducted in the study.Results：There were three immunoreactively-labeled neurons in the ganglia.The ER-staining was showed brown in color,locating in the nucleus;IL-6 staining was showed red in color,locating in the cytoplasm;there were ER/ IL-6 double-labeled cells in same section,which accounted for about 20% to 30 %of the total single and double labeled neurons.Conclusion：ER and IL-6 can coexist in same rat intra-cardiac ganglia cells.Therefore,it provides morphological evidence for the theory about immune-neuro-endocrine network at the cellular level.