羧甲基-β-环糊精作为手性选择剂对毛细管电泳手性拆分西酞普兰的影响

Chiral separation of Citalopram by capillary electrophoresis with carboxymethyl-β-cyclodextrin as chiral selector

目的以羧甲基-β-环糊精作为手性选择剂,研究西酞普兰的毛细管电泳手性拆分方法。方法选择和优化了手性选择剂的浓度、缓冲溶液的浓度、pH及分离电压等。电泳石英毛细管柱内径为75μm,总长65 cm,有效长度5 cm;紫外检测波长200 nm;柱温15℃。结果在含0.25%羧甲基-β-环糊精和25 mmol.L-1磷酸盐(pH7.0)的缓冲溶液中,分离电压为30kV时,西酞普兰对映体达到基线分离,分离度为1.6。结论所建方法可以分离分析西酞普兰对映体。

OBJECTIVE To study the chiral separation of citalopram based on capillary zone electrophoresis,with a fusedsilica capil- lary and carboxymethyl -β- cyclodrextrin as chiral selector. METHODS The effects of type and concentration of chiral selector, concentration and pH of background electrolyte, separation voltage used on the separation were investigated respectively. The separation was performed at 15 ℃ on a fused -silica capillary of 75μm × 65 em, effective length of 55 cm. The detection wavelength was 200 nm. Sample was injected into capillary at 0.5 p. s. i. for 5 s. RESULTS In the running phosphate buffer （25 mmol. L- 1 ） containing 0. 25% carboxymethyl-β- cyclodrextrin（ pHT. 0）,and at the applied high voltage of 30 kV,the citalopram＇ s enantiomers could be well separated. The resolution was 1.6. CONCLUSION The good resolution of citalopram enantiomers could be achieved under the optimal operation conditions.