HPLC测定痔血胶囊中的苦参碱

Determination of matrine in Zhixue capsules by HPLC

目的采用HPLC法测定痔血胶囊中苦参碱的含量。方法采用ZorbaxSB—C18色谱柱（250mm×4．6mm，5μm），流动相为甲醇-乙腈-磷酸盐缓冲液（pH6．8）一三乙胺（18：18：70：0．1），流速1．0ml·min-1，检测波长215nm。结果苦参碱的标准曲线为：Y=1．898×10^5X＋2．036×10^4（r=0．9999）在0．392～1．960μg的线性关系良好。结论所建方法简便、准确，可用于制剂的质量控制。

OBJECTIVE To develop an HPLC method for determination of matrine in Zhixue Capsules. METHODS The Zorbax SB - Ct8 column （250 mm×4. 6 mm , 5μm ）was used. The mobile phase consisted of methanol - acetonitrile - phosphate salt buffer solution （pH6.8） - triethylamine （18 ： 18 ： 70 ： 0. 1 ）. The flow rate was 1.0 ml-min-1. The detection wavelengh was at 215 nm. RESULTS There was a good linear relationship within the range of 0. 392 - 1. 960 μg, and regression equation was Y = 1. 898 × 10^5X ＋ 2. 036 x 10^4 （ r = 0. 9999）. CONCLUSION This method is simple and accurate, and it can be applied to the quality control of Zhixue capsule.