摘要
目的采用HPLC法测定仙灵止渴丸中的黄芪甲苷和柴胡皂苷a,d。方法采用EclipseXDBC,。色谱柱(250mm×4.6mm,5μm),以乙腈-水(33:67)为流动相,检测波长203nm。结果黄芪甲苷19.7—157.4μg·ml-1(r=0.9991)、柴胡皂苷a21.0—168.0μg·ml-1(r=0.9995)、柴胡皂苷d22.8~182.4μg·ml-1(r=0.9990)与峰面积的线性关系良好,平均回收率分别为99.2%(RSD=0.55%)、98.7%(RSD=0.73%)、99.3%(RSD=0.39%)。结论所建方法简便,灵敏度高,重复性好,可用于仙灵止渴丸的质量控制。
OBJECTIVE To establish a method for determination of Astragaloside IV and saikosaponin a, d in Xianling Zhike pills. METHODS Agilent Eclipse XDB -C18 (250 mm × 4.6 mm,5μm)column was used, the mobile phase was acetonitrile -water(33 : 67 ) and detection wavelength was at 203 nm. RESULTS The calibration curve was linear in the range of 19.7 - 157.4 μg· ml -1 ( r = 0.9991 ) for astragaloside IV, 21.0 - 168.0 μg· ml - 1 ( r = 0. 9995 ) for saikosaponin a and 22.8 - 182.4 μg· ml -1 ( r = 0. 9990 ) for saikosaponined. The average recoveries were 99.2% (RSD = 0.55% ) for astragaloside IV, 98.7% (RSD = 0.73% ) for saikosaponin a and 99.3% ( RSD = 0.39% ) for saikosaponind. CONCLUSION The method is simple, accurate and can be used to control the qual- ity of Xianling Zhike pills.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第5期608-609,共2页
West China Journal of Pharmaceutical Sciences