Z24对大鼠肝脏CYP450酶系的影响

Effects of Z24 on rat liver cytochrome P450 system

目的:观察Z24对大鼠肝脏CYP450酶系的影响。方法:雌性Wistar大鼠,灌胃(ig)给予Z24(0,50,100,200 mg.kg-1.d-1),连续5 d,以0.9%氯化钠溶液作对照,末次给药后次日,处死大鼠,测定肝微粒体中CYP450总含量、细胞色素b5(Cyt-b5)含量、NADPH-CYP450还原酶活性以及1A2,1B1,2E1,3A4亚型活性。结果:与对照组相比,各剂量组CYP450总含量、NADPH-CYP450还原酶活性及CYP1B1、2E1亚型活性均明显升高(P<0.05);100和200 mg.kg-1组Cyt-b5含量升高(P<0.05);200 mg.kg-1组CYP1A2和3A亚型活性升高(P<0.05)。结论:Z24对CYP450、NADPH-CYP450还原酶及CYP1B1、2E1亚型有诱导作用,剂量达100和200 mg.kg-1时分别对Cyt-b5和CYP1A2、3A亚型产生诱导作用。

Objective： To investigate the effect of Z24 on the contents and activities of cytoehrome P450 （CYP450） system in rat liver. Methods： In female Wistar rats, Z24 （50, 100 and 200 mg·kg^-1 ） or saline （control） was intragastrically administrated for 5 days. Then, total contents of CYP450 and cytoehrome b5, and activities of NADPH-CYP450 reductase and the subtypes CYP1A2,1B1,2E1 ,3A in liver microsome were detected. Results： Total CYP450 contents, activities of NADPH-CYP450 reductase and subtype CYP1B1, 2E1 were higher in all three Z24 groups than in control group. Cytoehrome b5 content increased in Z24 100 and 200 mg·kg^-1 groups （P〈0.05）, and the activities of subtypes CYP1A2 and CYP 3A increased in Z24 200 mg·kg^-1 group （P 〈 0.05） Conclusion： CYP450, NADPH-CYP450 reductase and subtypes CYP1B1, 2E1 can be induced by Z24. Cytochtome, b5. and subtypes 1A2 and 3A can also be induced by Z24 at 100 and 200 mg.kg^-1