人参皂苷Rh2两种差向异构体的HPLC含量测定

Determination of two ginsenoside Rh_2 by HPLC

目的：建立2种人参皂苷Rh2异构体的高效液相色谱测定方法。方法：采用Merck Lichro-spherRP-18e C18色谱柱（250mm×4．6mm，5μm），流动相为CH3CN—CH3OH（65：35），检测波长为203nm，流速为1．0mL·min^-1。结果：Rh2线性范围为0．06～1．20g·L^-1，以峰面积（y）对浓度（x，g·L^-1）求得线性方程，S构型线性方程为y=2260．9x-12．533，r=0．9999；R构型线性方程为Y=2596．1x-7．9654，r=0．9999。结论：本方法简便、准确、可靠，可用于Rh2中的2种差向异构体化合物的鉴别和含量测定。

Objective ： To establish a method for the determination of two ginsenoside Rh2 by HPLC. Methods：Analytical column was Merck LichrospherRP-18e C18 （250 mm ×4.6 mm,5 μm） ,and the mobile phase consisted of CH3CN-CH3OH（65： 35）. The detection wavelength was set at 203 nm,and the flow rate was 1.0 mL·min^ -1. Results ： Rh2 showed a linear relationship in the concentration range from 0.06 g·L^-1 to 1.20 g·L^-1. The equation of S-Rh2 is y =2 260.9x - 12. 533,r = 0. 999 9; the equation of R-Rh2 is y = 2 596.1x - 7. 965 4,r = 0. 999 9. Conclusion：The method is simple and suitable for assay of R-Rh2 and S-Rh2.