高效液相色谱法测定调肝和胃丸中芍药苷的含量

Determination of Paeoniflorin in Tiaoganhewei Pill by HPLC

目的:采用高效液相色谱法(HPLC),测定调肝和胃丸中芍药苷的含量,以控制该制剂的质量。方法:高效液相色谱法,色谱柱为C18柱,VP-ODS1 50L×4.6。流动相为乙腈-0.1%磷酸(18∶82),流速1.0ml/min,柱温为25℃,检测波长为230nm。结果:精密度和稳定性均良好;芍药苷峰与其它组分峰的分离度为3.6,理论塔板数以芍药苷峰计算为8563,平均加样回收率为99.2%(RSD为1.08%,n=5),芍药苷在0.25~2.5μg范围内进样量与吸收面积值呈良好的线性关系。结论:用HPLC法测定调肝和胃丸中芍药苷的含量,结果准确、重现性好。

Objective： To test and control the quality of the pill by the determination of paeoniflorin in Tiaoganhewei Pill, using the method of HPLC. Methods： Use HPLC method with a C18 column, VP-ODS 150L×4.6, and a mixture of Acetonitrile-0. 1% Phosphoric acid（18：82） as the mobile phase. The flowing rate is 1.0ml/min. The temperature of the column is 25℃ and the detecting wavelength is 230nm. Results： The precision and stability were fine. Resolution between paeoniflorin and other peaks was 3.6. The theoretic plate number was figured out as 8563 based on the peak of paeoniflorin. The average recovery of paeoniflorin was 99.2 % with RSD 1.08 % （ n = 5）. The injection volume of paeoniflorin and the absorption area pre^nted a good linear response, in a range of 0.25-.5ptg of paeoniflorin. Conclusion ： The method of HPLC in the determination of paeoniflorin was precise and presented good reproducibility.