应用微重力旋转培养系统构建组织工程软骨的实验研究

Experimental cartilage engineering with microgravity rotating cell culture system

目的探讨微重力旋转培养系统(RCCS)在构建组织工程细胞/载体复合物修复兔关节软骨缺损中的作用。方法将兔骨髓间充质干细胞附着在三维载体壳聚糖上,分为传统的静置培养组和微重力旋转培养组,经过体外成软骨诱导作用后,修复兔关节软骨缺损。考察不同时间点、不同培养体系中细胞增殖情况、成软骨表达情况以及对兔关节软骨缺损的修复情况。结果相对于常规的静置培养组,RCCS组细胞增殖能力、成软骨表达均得到增强,对兔关节软骨缺损的修复更为有效。结论微重力旋转培养系统可以作为构建组织工程软骨一个有效的辅助手段,进而应用临床软骨缺损的修复。

Objective To explore the role of mierogravity rotating cell culture system （RCCS） in the cartilage tissue-engineering for repair of cartilage defects. Methods Rabbit marrow derived mesenchymal stem cell （MSCs） attached to chitosan （as the three dimensional vector） were divided into two groups, one cultured in the mierogravity rotating culture system and the other cultured in tranditional static culture system, both exposed to chondrogenic conditions. After two weeks induction, the cell-vector complexes were transplanted to repair the cartilage defects created in rabbit femur as allografts. The proliferation, chondrogenesis of MSCs and outcomes of cartilage repair were compared between the two groups. Results Compared with those in static culture system, proliferation and chondrogenesis of MSCs in RCCS were more intense, and the outcomes of repair with RCCS-derived cartilage appeared more reliable. Conclusion The RCCS can be used as an effective tool for cartilage tissure-enigeering.