川东獐牙菜中1,8-二羟基-3,7-二甲氧基酮含量测定研究

Quantitative Determination of 1,8-Dihydroxy-3,7-Dimethoxyxanthones in Swertia davidi Franch by HPLC

目的采用高效液相色谱法测定川东獐牙菜中1,8-二羟基-3,7-二甲氧基酮的含量。方法色谱柱为Hypersil C18柱(150mm×4.6mm,5μm),流动相为甲醇-水(60∶40),检测波长254nm,流速1mL/min,柱温室温。结果1,8-二羟基-3,7-二甲氧基酮线性范围为0.18～1.80μg,r=0.9998(n=5),平均加样回收率为100.15%,RSD=2.04%(n=9)。结论该方法简便、快速,适用于川东獐牙菜的含量测定。

Objective To determine the content of 1, 8- dihydroxy-3, 7-dimethoxyxanthones in Swertia davidi Franch by HPLC. Method The analysis was carried out on Shimadzu C18 column （150 mm×4.6 mm,5 μm）.The mobile phase was MeOH-H2O （60：40）.The wave- length was 254 nm. The flowrate was 1.0 mL/min. The temperature was room temperature. Results The liner range of 1, 8-dihydroxy- 3, 7-dimethoxyxanthones was 0.18-1.80 μg,r=0.999 8（n=5）.The recovery rate was 100.15%,RSD=2.04%（n=9）. Conclusion The method is simple and fast with good linear relationship. It can be used to control the quality.