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抗人Cx31第234位磷酸丝氨酸抗体的制备

Preparation of the 234th Phosphserine of the Cx31 Antibody
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摘要 利用磷酸化多肽合成技术合成Cx31羧基端磷酸多肽片段(CSPSSSApSRAST),经HPLC纯化、质谱证实后偶联到匙孔槭血蓝蛋白,免疫新西兰雄兔后采血检测、并经过特异性纯化、经Western blotting检测,证实得到的抗体为抗间隙连接蛋白31第234位丝氨酸的特异抗体. The peptide-KLH solution was mixed with freund＇s complete or incomplete adjuvant and immunized rabbit to prepare specific polyclonal antibody against the topology of connexin31. A full-length human connexin31 gene was cloned into expression vector, pcDNA3.1/myc-his（-）B, and transformed into HT 1080 cell line stably. The expressed protein was used to ensure the polyclonal antibody. Western blotting analysis suggested that the polyclonal antibody recognize the fusion protein of the connexin31-myc. The antiserum against connexin31 prepared by differential immunogenic peptide-KLH has good specificity.
作者 寻晓红 刘宇
机构地区 湖南理工学院化学化工系
出处 《湖南理工学院学报（自然科学版）》 CAS 2008年第3期52-55,共4页 Journal of Hunan Institute of Science and Technology(Natural Sciences)
基金 湖南省教育厅青年基金项目(07B029)
关键词 Cx31 磷酸多肽合成 WESTERN BLOTTING connexin31 phosph-peptide synthesis western blotting
分类号 TQ936 [化学工程]
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参考文献10

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湖南理工学院学报（自然科学版）
2008年 第3期

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