转CryIA和CpTI双价抗虫基因大豆的获得与稳定表达

Synchronous Expression of CryIA and CpTI Genes in Soybean and Analysis of Their Resistance to Insect Pests

【目的】将抗虫基因转入栽培大豆中,提高大豆的抗食心虫能力。【方法】利用大豆未成熟子叶体细胞胚发生系统高效转化体系,将携带有人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因植物表达载体pGBI121S4ABC转化到大豆主栽品种吉林20与吉林27中。【结果】GUS活性分析、PCR、Southern blot检测证明,目的基因已整合到受体大豆基因组中。T1代转基因大豆抗虫测定表明,转基因材料虫食率比对照显著降低,抗虫能力显著提高。【结论】T3～T5连续3代接虫测定结果表明,转基因大豆株系0-195和0-150的虫食率均比对照低大约30%,其抗虫能力已基本稳定。

[Objective] The objective of this study is to obtain highly insect-resisant transgenic soybean plants containing both Cryla and CpTI genes. [Method] Bivalent insect resistant genes CrylA and CpTI were transformed into immature cotyledon of soybean varieties JILIN 20 and JILIN 27 which have high frequency of somatic embryogenesis via Agrobacterium-mediated. [Result] GUS analysis, PCR and Southern blot analysis showed that the foreign gene was integrated into the chromosomal genome of receptor parent. [ Conclusion ] Analysis of insect resistance of successive three generations of transgenic soybean indicated that their resistance has been basically stable.